Traditional information asymmetry theories suggest that greater corporate transparency should reduce investor disagreement. Using Chinese mutual fund holdings, we document the opposite pattern: transparency amplifies disagreement among institutional investors. Mechanism tests show that transparency discourages herding while intensifying private information acquisition among fund managers. The effect is stronger for growth-oriented and high-skill funds, and during periods of elevated market sentiment, and among firms with lower credibility, excessive disclosure frequency, and greater investor attention. Further analysis indicates that this transparency-induced disagreement stems from informed trading rather than noise, thereby enhancing price informativeness and market efficiency. Overall, the evidence reveals the dual nature of transparency as both an informational input and a behavioral catalyst that increases disagreement in financial markets.