Based on the perspective of human capital and technology R & D regulation, this paper adopts the unexpected output super efficiency SBM model to calculate the ecological efficiency of digital enterprises in 30 provinces in China from 2010 to 2019, analyzes its spatial correlation, and empirically explores the driving factors of the ecological efficiency of Chinese digital enterprises under the role of environmental regulation. The results show that during the research period, the overall level of ecological efficiency of digital enterprises in China can be divided into three stages: gentle stage (2010-2014), trough stage (2014-2017) and fluctuation stage (2017-2019), and the eastern region > the central region > the western region; The ecological efficiency of China's digital enterprises as a whole shows a ladder like evolution law of decreasing from southeast to northwest, with significant spatial agglomeration and "block" characteristics; Environmental regulation has a lag effect on the ecological efficiency of digital enterprises, and the lag period inhibits the improvement of the ecological efficiency of digital enterprises; The level of human capital and the level of scientific and technological research and development have significant threshold characteristics. When the level of human capital is used as a threshold variable, the impact of environmental regulation on the ecological efficiency of digital enterprises is "U" shaped. When the level of scientific and technological research and development exceeds a certain threshold, environmental regulation has a negative impact on the ecological efficiency of digital enterprises.